View of Acetaminophen, the missing anion gap in the patient with metabolic acidosis The Southwest journal of medicine Silymarin prevents acetaminophen induced hepatotoxicity via up regulation of the glutathione conjugation capacity in mice ScienceDirect Acetaminophen induces liver injury and depletes glutathione in mice brain: Prevention by Moringa oleifera extract ScienceDirect Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.