Tylenol's Impact on Glutathione and Liver Damage Tylenol, Autism, And The Glutathione Connection OC Integrative Medicine The biochemistry of acetaminophen hepatotoxicity and rescue: a mathematical model Theoretical Biology and Medical Modelling Springer Nature Link Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.