Acetaminophen or Tylenol Toxicity Lect 19 Pharmacology PDF) A review of the evidence concerning hepatic glutathione depletion and susceptibility to liver toxicity after paracetamol overdose Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Acetaminophen metabolism