Can Tylenol (acetaminophen) deplete glutathione levels in the body? Not feeling well? *Don't* reach for Tylenol! Here are 8 reasons why. Some experts maintain that if acetaminophen were a brand new drug up for approval today, it would *not* get approved at Tylenol's Impact on Glutathione and Liver Damage Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.